The virus-host interaction during HBV infection determines infection outcome. However, current therapies using interferon or direct-acting antiviral agents are unlikely to eradicate the virus or cure the infection ( Gish et al., 2012 Revill et al., 2016) thus, efficient therapeutic approaches are urgent requirements. More than 200 million people are persistently infected with the hepatitis B virus (HBV) worldwide, and are at high risk of developing liver cirrhosis and hepatocellular carcinoma ( El-Serag, 2012 Trepo et al., 2014). Taken together, our findings provide a better understanding of the antibody repertoires of HBV chronically infected individuals.Ĭhronic hepatitis B is a major global health issue. These results indicated that low level of serum HBV might not induce significant changes in BCR repertoires, and high level of HBV replication could have more impacts on IgM repertories than IgG repertoires. In contrast, for IgG repertories, the preferred used VDJ genes were similar in all the three populations. Besides, the biased used IGHD genes were IGHD2-2 and IGHD3-3 in CHB library but were IGHD3-10 and IGHD3-22 in IHB and HH library. More shared antibody clones were found between the IgM repertoires of IHB and HH than that found between CHB and HH (7079 clones vs. Such difference in clone diversity and expansion was not observed in the IgG repertoires of the three populations. Nevertheless, the diversity of the unique clones decreased and some clusters of unique clones expanded in the IgM repertoire of chronic HBV carriers (CHB) compared with healthy adults (HH) and inactive HBV carriers (IHB). The comparative study revealed high levels of similarity between the IgM and IgG repertoires of the HBV carriers and the healthy adults, including the somatic mutations in V regions, the average CDR3 length, and the occurrence of junctional modifications. To investigate the characteristics of antibody repertoires in patients with chronic HBV infection, we performed Illumina sequencing and IMGT/HighV-QUEST analysis of B lymphocytes from healthy adults and the HBV carriers with high or low level of viral replication. High-throughput antibody sequencing allows in-depth insights into human antibody repertoires. 3Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.2Traditional Chinese Medicine Department, Rehabilitation Hospital, Quanzhou, China.1Central Laboratory, Second Affiliated Hospital, Fujian Medical University, Quanzhou, China.Phone: 404.712.2970 Email: note: SG, DCN, and JLH are co–first authors.Binbin Hong 1*, Lizhi Wang 2, Chunlan Huang 2, Xiaoju Hong 2, Alan Liu 2, Qiulan Li 1, Qiaoling Liu 1, Lili Su 1, Lixing Wang 1, Chunyu Wang 3 and Tianlei Ying 3* Eun-Hyung Lee, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, Emory University, 615 Michael Street, Suite 205, Atlanta, Georgia 30322, USA. Craig Venter Institute, La Jolla, California, USA.Īddress correspondence to: F. 1School of Biological Sciences, Georgia Institute of Technology, andĢDivision of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Emory University, Department of Medicine, Atlanta, Georgia, USA.ģFinger Lakes Community College, Rochester, New York, USA.ĤDivision of Rheumatology, Emory University, andĥLowance Center for Human Immunology in the Departments of Medicine and Pediatrics at Emory University, Atlanta, Georgia, USA.ĦDepartment of Microbiology and Informatics Institute, University of Alabama, Birmingham, Alabama, USA.ħInformatics Institute, University of Alabama Birmingham, Alabama, USA.ĨUniversity of Rochester, Rochester, New York, USA.ĩJ.
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